Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library.
Identifieur interne : 001F23 ( Main/Exploration ); précédent : 001F22; suivant : 001F24Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library.
Auteurs : Anizah Rahumatullah [Malaisie] ; Azimah Ahmad [Malaisie] ; Rahmah Noordin [Malaisie] ; Theam Soon Lim [Malaisie]Source :
- Molecular immunology [ 1872-9142 ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (génétique), Anticorps antihelminthe (immunologie), Anticorps monoclonaux (immunologie), Anticorps à chaîne unique (), Anticorps à chaîne unique (génétique), Anticorps à chaîne unique (immunologie), Antigènes d'helminthe (immunologie), Banque de peptides, Brugia malayi (immunologie), Cellules germinales (métabolisme), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Humains, Immunisation, Régions déterminant la complémentarité (immunologie), Séquence d'acides aminés, Test ELISA.
- MESH :
- génétique : Anticorps antihelminthe, Anticorps à chaîne unique.
- immunologie : Anticorps antihelminthe, Anticorps monoclonaux, Anticorps à chaîne unique, Antigènes d'helminthe, Brugia malayi, Filariose lymphatique, Régions déterminant la complémentarité.
- métabolisme : Cellules germinales.
- parasitologie : Filariose lymphatique.
- Animaux, Anticorps à chaîne unique, Banque de peptides, Humains, Immunisation, Séquence d'acides aminés, Test ELISA.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Helminth (genetics), Antibodies, Helminth (immunology), Antibodies, Monoclonal (immunology), Antigens, Helminth (immunology), Brugia malayi (immunology), Complementarity Determining Regions (immunology), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Enzyme-Linked Immunosorbent Assay, Germ Cells (metabolism), Humans, Immunization, Peptide Library, Single-Chain Antibodies (chemistry), Single-Chain Antibodies (genetics), Single-Chain Antibodies (immunology).
- MESH :
- chemical , chemistry : Single-Chain Antibodies.
- chemical , genetics : Antibodies, Helminth, Single-Chain Antibodies.
- chemical , immunology : Antibodies, Helminth, Antibodies, Monoclonal, Antigens, Helminth, Complementarity Determining Regions, Single-Chain Antibodies.
- immunology : Brugia malayi, Elephantiasis, Filarial.
- metabolism : Germ Cells.
- parasitology : Elephantiasis, Filarial.
- Amino Acid Sequence, Animals, Enzyme-Linked Immunosorbent Assay, Humans, Immunization, Peptide Library.
Abstract
Phage display technology is an important tool for antibody generation or selection. This study describes the development of a scFv library and the subsequent analysis of identified monoclonal antibodies against BmSXP, a recombinant antigen for lymphatic filariasis. The immune library was generated from blood of lymphatic filariasis infected individuals. A TA based intermediary cloning approach was used to increase cloning efficiency for the library construction process. A diverse immune scFv library of 10(8) was generated. Six unique monoclonal antibodies were identified from the 50 isolated clones against BmSXP. Analysis of the clones showed a bias for the IgHV3 and Vκ1 (45.5%) and IgHV2 and Vκ3 (27.3%) gene family. The most favored J segment for light chain is IgKJ1 (45.5%). The most favored D and J segment for heavy chain are IgHD6-13 (75%) and IgHJ3 (47.7%). The information may suggest a predisposition of certain V genes in antibody responses against lymphatic filariasis.
DOI: 10.1016/j.molimm.2015.07.040
PubMed: 26277276
Affiliations:
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Le document en format XML
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Electronic address: theamsoon@usm.my.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Penang</wicri:regionArea><wicri:noRegion>Penang</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular immunology</title><idno type="eISSN">1872-9142</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Helminth (genetics)</term><term>Antibodies, Helminth (immunology)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antigens, Helminth (immunology)</term><term>Brugia malayi (immunology)</term><term>Complementarity Determining Regions (immunology)</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Germ Cells (metabolism)</term><term>Humans</term><term>Immunization</term><term>Peptide Library</term><term>Single-Chain Antibodies (chemistry)</term><term>Single-Chain Antibodies (genetics)</term><term>Single-Chain Antibodies (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (génétique)</term><term>Anticorps antihelminthe (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Anticorps à chaîne unique ()</term><term>Anticorps à chaîne unique (génétique)</term><term>Anticorps à chaîne unique (immunologie)</term><term>Antigènes d'helminthe (immunologie)</term><term>Banque de peptides</term><term>Brugia malayi (immunologie)</term><term>Cellules germinales (métabolisme)</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (parasitologie)</term><term>Humains</term><term>Immunisation</term><term>Régions déterminant la complémentarité (immunologie)</term><term>Séquence d'acides aminés</term><term>Test ELISA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Single-Chain Antibodies</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antibodies, Helminth</term><term>Single-Chain Antibodies</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antibodies, Monoclonal</term><term>Antigens, Helminth</term><term>Complementarity Determining Regions</term><term>Single-Chain Antibodies</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Anticorps à chaîne unique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Anticorps monoclonaux</term><term>Anticorps à chaîne unique</term><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Filariose lymphatique</term><term>Régions déterminant la complémentarité</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Germ Cells</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules germinales</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Immunization</term><term>Peptide Library</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Anticorps à chaîne unique</term><term>Banque de peptides</term><term>Humains</term><term>Immunisation</term><term>Séquence d'acides aminés</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phage display technology is an important tool for antibody generation or selection. This study describes the development of a scFv library and the subsequent analysis of identified monoclonal antibodies against BmSXP, a recombinant antigen for lymphatic filariasis. The immune library was generated from blood of lymphatic filariasis infected individuals. A TA based intermediary cloning approach was used to increase cloning efficiency for the library construction process. A diverse immune scFv library of 10(8) was generated. Six unique monoclonal antibodies were identified from the 50 isolated clones against BmSXP. Analysis of the clones showed a bias for the IgHV3 and Vκ1 (45.5%) and IgHV2 and Vκ3 (27.3%) gene family. The most favored J segment for light chain is IgKJ1 (45.5%). The most favored D and J segment for heavy chain are IgHD6-13 (75%) and IgHJ3 (47.7%). The information may suggest a predisposition of certain V genes in antibody responses against lymphatic filariasis.</div></front></TEI><affiliations><list><country><li>Malaisie</li></country></list><tree><country name="Malaisie"><noRegion><name sortKey="Rahumatullah, Anizah" sort="Rahumatullah, Anizah" uniqKey="Rahumatullah A" first="Anizah" last="Rahumatullah">Anizah Rahumatullah</name></noRegion><name sortKey="Ahmad, Azimah" sort="Ahmad, Azimah" uniqKey="Ahmad A" first="Azimah" last="Ahmad">Azimah Ahmad</name><name sortKey="Lim, Theam Soon" sort="Lim, Theam Soon" uniqKey="Lim T" first="Theam Soon" last="Lim">Theam Soon Lim</name><name sortKey="Noordin, Rahmah" sort="Noordin, Rahmah" uniqKey="Noordin R" first="Rahmah" last="Noordin">Rahmah Noordin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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